BAIT
IRF8
AI893568, ICSBP, IRF-8, Icsbp1, Myls
interferon regulatory factor 8
GO Process (13)
GO Function (3)
GO Component (2)
Gene Ontology Biological Process
- cellular response to lipopolysaccharide [IMP]
- defense response to bacterium [IMP]
- defense response to protozoan [IMP]
- immune response [IMP]
- myeloid cell differentiation [IMP]
- negative regulation of growth of symbiont in host [IMP]
- phagocytosis [IMP]
- positive regulation of interferon-gamma production [IMP]
- positive regulation of interleukin-12 production [IMP]
- positive regulation of transcription initiation from RNA polymerase II promoter [IDA]
- positive regulation of transcription, DNA-templated [IPI]
- regulation of transcription, DNA-templated [TAS]
- response to bacterium [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- nucleoplasm [ISO]
- nucleus [IDA]
Mus musculus
PREY
SUMO1
GMP1, PIC1, SENTRIN, SMT3, SMT3H3, SMTP3, SUMO-1, Smt3C, Ubl1
SMT3 suppressor of mif two 3 homolog 1 (yeast)
GO Process (14)
GO Function (5)
GO Component (10)
Gene Ontology Biological Process
- PML body organization [IMP]
- negative regulation of DNA binding [ISO]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- palate development [IMP]
- positive regulation of calcium-transporting ATPase activity [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein localization to nuclear pore [IMP]
- protein sumoylation [IDA, IMP, ISO]
- regulation of calcium ion transmembrane transport [IMP]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of protein stability [IMP]
- regulation of transcription, DNA-templated [IDA]
Gene Ontology Molecular Function
Mus musculus
Two-hybrid
Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.
Publication
Ubc9-mediated sumoylation leads to transcriptional repression of IRF-1.
To characterize the regulatory mechanism of the interferon regulatory factor (IRF) family, we performed yeast two-hybrid screening with IRF-2 and isolated the small ubiquitin-related modifier (SUMO)-conjugating enzyme Ubc9, which also interacts with other IRF family members IRF-1 and ICSBP. Subsequent assays indicated that among the IRF family members, only IRF-1 interacts with SUMO-1 through its transcriptional activation domain. The interaction ... [more]
Biochem. Biophys. Res. Commun. Dec. 19, 2008; 377(3);952-6 [Pubmed: 18955028]
Throughput
- Low Throughput
Additional Notes
- #LPPI
- Likely protein-protein interaction
Curated By
- BioGRID