SUMO1
Gene Ontology Biological Process
- PML body organization [IMP]
- negative regulation of DNA binding [ISO]
- negative regulation of sequence-specific DNA binding transcription factor activity [ISO]
- negative regulation of transcription, DNA-templated [ISO]
- palate development [IMP]
- positive regulation of calcium-transporting ATPase activity [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [ISO]
- positive regulation of protein complex assembly [ISO]
- protein localization to nuclear pore [IMP]
- protein sumoylation [IDA, IMP, ISO]
- regulation of calcium ion transmembrane transport [IMP]
- regulation of cardiac muscle cell contraction [IMP]
- regulation of protein stability [IMP]
- regulation of transcription, DNA-templated [IDA]
Gene Ontology Molecular Function
IRF1
Gene Ontology Biological Process
- CD8-positive, alpha-beta T cell differentiation [IMP]
- I-kappaB kinase/NF-kappaB signaling [ISO]
- apoptotic process [ISO]
- cell cycle arrest [ISO]
- cellular response to interferon-beta [ISO]
- cellular response to interleukin-1 [ISO]
- cellular response to peptide hormone stimulus [ISO]
- cellular response to tumor necrosis factor [ISO]
- defense response to virus [IMP, ISO]
- interferon-gamma-mediated signaling pathway [IMP]
- negative regulation of T-helper 2 cell differentiation [TAS]
- negative regulation of regulatory T cell differentiation [IMP]
- negative regulation of transcription, DNA-templated [IDA, ISO]
- negative regulation of tyrosine phosphorylation of Stat1 protein [ISO]
- positive regulation of T-helper 1 cell differentiation [TAS]
- positive regulation of cell death [ISO]
- positive regulation of interferon-beta production [ISO]
- positive regulation of interleukin-12 biosynthetic process [IDA, IMP]
- positive regulation of natural killer cell differentiation [TAS]
- positive regulation of transcription from RNA polymerase II promoter [IDA, ISO]
- positive regulation of transcription, DNA-templated [IDA, IMP, ISO]
- positive regulation of type I interferon production [IMP]
- regulation of CD8-positive, alpha-beta T cell proliferation [IMP]
- regulation of MyD88-dependent toll-like receptor signaling pathway [IMP]
- regulation of adaptive immune response [TAS]
- regulation of gene expression [IMP]
- regulation of innate immune response [TAS]
- response to growth hormone [ISO]
- transcription from RNA polymerase II promoter [ISO]
Gene Ontology Molecular Function- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- protein binding [IPI]
- sequence-specific DNA binding [IDA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Ubc9-mediated sumoylation leads to transcriptional repression of IRF-1.
To characterize the regulatory mechanism of the interferon regulatory factor (IRF) family, we performed yeast two-hybrid screening with IRF-2 and isolated the small ubiquitin-related modifier (SUMO)-conjugating enzyme Ubc9, which also interacts with other IRF family members IRF-1 and ICSBP. Subsequent assays indicated that among the IRF family members, only IRF-1 interacts with SUMO-1 through its transcriptional activation domain. The interaction ... [more]
Throughput
- Low Throughput
Additional Notes
- #LPPI
- Likely protein-protein interaction
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| IRF1 SUMO1 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | 696380 |
Curated By
- BioGRID