An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Histone deacetylase 8 safeguards the human ever-shorter telomeres 1B (hEST1B) protein from ubiquitin-mediated degradation.

Lee H, Sengupta N, Villagra A, Rezai-Zadeh N, Seto E

Histone deacetylases (HDACs) are enzymes that regulate the functions of histones as well as nonhistones by catalyzing the removal of acetyl groups from lysine residues. HDACs regulate many biological processes, including the cell division cycle and tumorigenesis. Although recent studies have implicated HDAC8 in tumor cell proliferation, the molecular mechanisms linking HDAC8 to cell growth remain unknown. Here, we report ... [more]

Mol. Cell. Biol. Jul. 01, 2006; 26(14);5259-69 [Pubmed: 16809764]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

HDAC8

Gene Ontology Biological Process

Gene Ontology Molecular Function

histone deacetylase activity [TAS]transcription factor binding [TAS]

Gene Ontology Cellular Component

HSPA4