Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Tetraspanin 6 (TSPAN6) negatively regulates RIG-I-like receptors (RLRs) mediated immune signaling in a ubiquitination dependent manner.

Wang Y, Tong X, Omoregie ES, Liu W, Meng S, Ye X

The recognition between RIG-I-like receptors (RLRs) and viral RNA triggers an intracellular cascade of signaling to induce the expression of type I interferons (IFNs). Both positive and negative regulation on RLRs signaling pathway are important for host anti-viral immune response. Here we demonstrated that tetraspanin protein TSPAN6 inhibits RLR signaling by affecting the formation of adaptor MAVS (mitochondria antiviral signaling) ... [more]

J. Biol. Chem. Aug. 20, 2012; 0(0); [Pubmed: 22908223]

Throughput

  • Low Throughput

Additional Notes

  • figure 4.

Curated By

  • BioGRID