An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Novel interaction partners of the CD2BP2-GYF domain.

Kofler M, Motzny K, Beyermann M, Freund C

The GYF domain of CD2BP2 serves as an adapter that recognizes proline-rich sequences in intracellular proteins. Although the T cell adhesion molecule CD2 and the core splicing protein SmB/B' were previously shown to interact with CD2BP2-GYF, we are now using a general approach to identify putative GYF domain target sites within the human proteome. The phage display-derived recognition motif for ... [more]

J. Biol. Chem. Sep. 30, 2005; 280(39);33397-402 [Pubmed: 16000308]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

CD2BP2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]ribonucleoprotein complex binding [IDA]

Gene Ontology Cellular Component

AKNA

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]

Gene Ontology Cellular Component

Reconstituted Complex

Publication

Novel interaction partners of the CD2BP2-GYF domain.

Throughput

Curated By

protein binding [IPI]
ribonucleoprotein complex binding [IDA]

RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA]