PREY

SCNM1

RP11-68I18.6
sodium channel modifier 1
GO Process (0)
GO Function (1)
GO Component (2)

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Protein microarray analysis identifies human cellular prion protein interactors.

Satoh J, Obayashi S, Misawa T, Sumiyoshi K, Oosumi K, Tabunoki H

To obtain an insight into the function of cellular prion protein (PrPC), we studied PrPC-interacting proteins (PrPIPs) by analysing a protein microarray.We identified 47 novel PrPIPs by probing an array of 5000 human proteins with recombinant human PrPC spanning amino acid residues 23-231 named PR209.The great majority of 47 PrPIPs were annotated as proteins involved in the recognition of nucleic ... [more]

Neuropathol. Appl. Neurobiol. Feb. 01, 2009; 35(1);16-35 [Pubmed: 18482256]

Throughput

  • Low Throughput

Additional Notes

  • Protein microarray analysis

Curated By

  • BioGRID