SORBS1
Gene Ontology Biological Process
- cellular response to insulin stimulus [IC, IMP]
- focal adhesion assembly [IDA]
- glucose transport [IDA]
- insulin receptor signaling pathway [IC, IDA]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glycogen biosynthetic process [IMP]
- positive regulation of lipid biosynthetic process [IMP]
- positive regulation of signal transduction [IPI]
- stress fiber assembly [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell-cell adherens junction [IDA]
- cell-substrate adherens junction [IDA]
- centrosome [ISO]
- cytoplasm [ISO]
- cytoskeleton [IDA]
- cytosol [IDA]
- flotillin complex [IC]
- focal adhesion [ISO]
- insulin receptor complex [ISO]
- membrane [IDA]
- membrane raft [IDA]
- nuclear matrix [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- stress fiber [IDA]
SH2B2
Gene Ontology Biological Process
- B-1 B cell homeostasis [IMP]
- actin cytoskeleton organization [IMP, IPI]
- antigen receptor-mediated signaling pathway [IDA]
- brown fat cell differentiation [IDA]
- cytokine-mediated signaling pathway [IDA]
- glucose homeostasis [ISA]
- insulin receptor signaling pathway [IDA]
- intracellular signal transduction [IDA, ISO]
- negative regulation of glucose import [ISA]
- nervous system development [ISO]
- positive regulation of signal transduction [IDA]
- regulation of JAK-STAT cascade [ISO]
- regulation of Ras protein signal transduction [ISO]
- regulation of immune response [IMP]
- regulation of metabolic process [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
APS facilitates c-Cbl tyrosine phosphorylation and GLUT4 translocation in response to insulin in 3T3-L1 adipocytes.
APS is a Cbl-binding protein that is tyrosine phosphorylated by the insulin receptor kinase. Insulin-stimulated phosphorylation of tyrosine 618 in APS is necessary for its association with c-Cbl and the subsequent tyrosine phosphorylation of Cbl by the insulin receptor in both 3T3-L1 adipocytes and CHO-IR cells. When overexpressed in these cells, wild-type APS but not an APS/Y(618)F mutant facilitated the ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 4.
Curated By
- BioGRID