BAIT

NPHP1

JBTS4, NPH1, SLSN1
nephronophthisis 1 (juvenile)
Homo sapiens
PREY

ARHGAP32

GC-GAP, GRIT, PX-RICS, RICS, p200RhoGAP, p250GAP
Rho GTPase activating protein 32
GO Process (2)
GO Function (1)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Nephrocystin-1 interacts directly with Ack1 and is expressed in human collecting duct.

Eley L, Moochhala SH, Simms R, Hildebrandt F, Sayer JA

Nephronophthisis is characterised by renal fibrosis, tubular basement membrane disruption and corticomedullary cyst formation leading to end stage renal failure. Mutations in NPHP1 account for the underlying genetic defect in 25% of patients with nephronophthisis. Loss of urine concentration ability may be an early feature of nephronophthisis. Using yeast-2-library screening with the SH3 domain of nephrocystin-1 as bait, we identify ... [more]

Biochem. Biophys. Res. Commun. Jul. 11, 2008; 371(4);877-82 [Pubmed: 18477472]

Throughput

  • Low Throughput

Additional Notes

  • table 2.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ARHGAP32 NPHP1
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
696871

Curated By

  • BioGRID