Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Actinfilin, a brain-specific actin-binding protein in postsynaptic density.

Chen Y, Derin R, Petralia RS, Li M

The dynamic assembly and disassembly of actin-based cytoskeleton is closely linked to the changes in the postsynaptic density in both number and shape, which is thought to be important in forming long-term memory. Thus, regulation of actin filaments may play a critical role in contributing to the formation of long-term memory. Here, we report the cloning of actinfilin, a brain-specific ... [more]

J. Biol. Chem. Aug. 23, 2002; 277(34);30495-501 [Pubmed: 12063253]

Throughput

  • Low Throughput

Additional Notes

  • Data not shown

Curated By

  • BioGRID