Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

CSN5/Jab1 inhibits cardiac L-type Ca2+ channel activity through protein-protein interactions.

Kameda K, Fukao M, Kobayashi T, Tsutsuura M, Nagashima M, Yamada Y, Yamashita T, Tohse N

L-type Ca(2+) channels have a wide tissue distribution and play essential roles in physiological responses. Recent studies have indicated that regulation of L-type Ca(2+) channels involves the assembly of macromolecular signaling complexes such as the beta(2)-adrenergic receptor signaling complex, the small G-protein kir/Gem and the BK channel. Here, we report the previously unidentified role of another protein in binding to ... [more]

J. Mol. Cell. Cardiol. Apr. 01, 2006; 40(4);562-9 [Pubmed: 16483597]

Throughput

  • Low Throughput

Curated By

  • BioGRID