PPP2R4
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- mitotic spindle organization in nucleus [IBA]
- negative regulation of phosphoprotein phosphatase activity [IDA]
- negative regulation of protein dephosphorylation [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of phosphoprotein phosphatase activity [IDA]
- positive regulation of protein dephosphorylation [IDA]
- protein peptidyl-prolyl isomerization [IBA]
- regulation of phosphoprotein phosphatase activity [IDA]
Gene Ontology Molecular Function- ATP binding [IDA]
- ATPase activity [IDA]
- peptidyl-prolyl cis-trans isomerase activity [IBA]
- protein heterodimerization activity [TAS]
- protein homodimerization activity [IDA]
- protein phosphatase 2A binding [IDA]
- protein phosphatase type 2A regulator activity [IDA, TAS]
- protein tyrosine phosphatase activator activity [IDA]
- receptor binding [IPI]
- ATP binding [IDA]
- ATPase activity [IDA]
- peptidyl-prolyl cis-trans isomerase activity [IBA]
- protein heterodimerization activity [TAS]
- protein homodimerization activity [IDA]
- protein phosphatase 2A binding [IDA]
- protein phosphatase type 2A regulator activity [IDA, TAS]
- protein tyrosine phosphatase activator activity [IDA]
- receptor binding [IPI]
Gene Ontology Cellular Component
CYLD
Gene Ontology Biological Process
- cytoplasmic translation [IBA]
- innate immune response [TAS]
- necroptotic process [IBA]
- negative regulation of NF-kappaB import into nucleus [IDA]
- negative regulation of NF-kappaB transcription factor activity [IDA]
- negative regulation of canonical Wnt signaling pathway [IMP]
- negative regulation of type I interferon production [TAS]
- nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway [TAS]
- nucleotide-binding oligomerization domain containing signaling pathway [TAS]
- positive regulation of extrinsic apoptotic signaling pathway [IMP]
- protein K63-linked deubiquitination [IDA]
- regulation of cilium assembly [ISS]
- regulation of intrinsic apoptotic signaling pathway [IMP]
- regulation of microtubule cytoskeleton organization [IMP]
- regulation of mitotic cell cycle [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Tumour suppressor CYLD is a negative regulator of the mitotic kinase Aurora-B.
The familial cylindromatosis tumour suppressor CYLD contains three cytoskeleton-associated protein glycine-rich (CAP-Gly) domains and a deubiquitinase domain. The tumour-suppressing function of CYLD has been attributed to its deubiquitinase domain, which removes lysine-63-linked polyubiquitin chains from target proteins, leading to the inhibition of cell survival and proliferation. In this study, we have detected an interaction of CYLD with the mitotic kinase ... [more]
Throughput
- Low Throughput
Additional Notes
- kinase activity inhibited in the presence of cyld
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PPP2R4 CYLD | Reconstituted Complex Reconstituted Complex An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator. | Low | - | BioGRID | 697413 |
Curated By
- BioGRID