SORBS1
Gene Ontology Biological Process
- cellular response to insulin stimulus [IC, IMP]
- focal adhesion assembly [IDA]
- glucose transport [IDA]
- insulin receptor signaling pathway [IC, IDA]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glycogen biosynthetic process [IMP]
- positive regulation of lipid biosynthetic process [IMP]
- positive regulation of signal transduction [IPI]
- stress fiber assembly [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell-cell adherens junction [IDA]
- cell-substrate adherens junction [IDA]
- centrosome [ISO]
- cytoplasm [ISO]
- cytoskeleton [IDA]
- cytosol [IDA]
- flotillin complex [IC]
- focal adhesion [ISO]
- insulin receptor complex [ISO]
- membrane [IDA]
- membrane raft [IDA]
- nuclear matrix [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- stress fiber [IDA]
CBL
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A novel, multifuntional c-Cbl binding protein in insulin receptor signaling in 3T3-L1 adipocytes.
The protein product of the c-Cbl proto-oncogene is prominently tyrosine phosphorylated in response to insulin in 3T3-L1 adipocytes and not in 3T3-L1 fibroblasts. After insulin-dependent tyrosine phosphorylation, c-Cbl specifically associates with endogenous c-Crk and Fyn. These results suggest a role for tyrosine-phosphorylated c-Cbl in 3T3-L1 adipocyte activation by insulin. A yeast two-hybrid cDNA library prepared from fully differentiated 3T3-L1 adipocytes ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 4.
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SORBS1 CBL | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | 696832 | |
| SORBS1 CBL | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID