INSR
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [ISO]
- activation of MAPK activity [ISO]
- activation of protein kinase B activity [ISO]
- activation of protein kinase activity [ISO]
- cellular response to growth factor stimulus [IDA]
- cellular response to insulin stimulus [ISO]
- epidermis development [IMP]
- exocrine pancreas development [IMP]
- glucose homeostasis [ISO]
- heart morphogenesis [ISO]
- insulin receptor signaling pathway [IMP, ISO]
- intracellular signal transduction [ISO]
- male sex determination [IMP]
- negative regulation of gene expression [ISO]
- negative regulation of protein phosphorylation [ISO]
- negative regulation of transporter activity [ISO]
- organ morphogenesis [IMP]
- peptidyl-tyrosine autophosphorylation [IDA]
- peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of DNA replication [ISO]
- positive regulation of MAPK cascade [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of cell proliferation [ISO]
- positive regulation of developmental growth [ISO]
- positive regulation of glucose import [ISO]
- positive regulation of glycogen biosynthetic process [IMP, ISO]
- positive regulation of glycolytic process [ISO]
- positive regulation of glycoprotein biosynthetic process [ISO]
- positive regulation of mitosis [IMP, ISO]
- positive regulation of nitric oxide biosynthetic process [ISO]
- positive regulation of phosphorylation [ISO]
- positive regulation of protein kinase B signaling [ISO]
- positive regulation of protein phosphorylation [ISO]
- positive regulation of respiratory burst [ISO]
- protein autophosphorylation [ISO]
- protein heterotetramerization [ISO]
- protein phosphorylation [ISO]
- regulation of embryonic development [ISO]
- regulation of hydrogen peroxide metabolic process [ISO]
- regulation of transcription, DNA-templated [ISO]
- response to tumor necrosis factor [ISO]
- signal transduction by phosphorylation [ISO]
- transformation of host cell by virus [ISO]
Gene Ontology Molecular Function- 3-phosphoinositide-dependent protein kinase binding [ISO]
- ATP binding [ISO]
- GTP binding [ISO]
- PTB domain binding [ISO]
- insulin binding [ISO]
- insulin receptor substrate binding [ISO]
- insulin-activated receptor activity [ISO]
- insulin-like growth factor I binding [ISO]
- insulin-like growth factor II binding [ISO]
- insulin-like growth factor receptor binding [ISO]
- lipoic acid binding [ISO]
- phosphatidylinositol 3-kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein kinase activity [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- protein tyrosine kinase activity [ISO]
- receptor signaling protein tyrosine kinase activity [ISO]
- 3-phosphoinositide-dependent protein kinase binding [ISO]
- ATP binding [ISO]
- GTP binding [ISO]
- PTB domain binding [ISO]
- insulin binding [ISO]
- insulin receptor substrate binding [ISO]
- insulin-activated receptor activity [ISO]
- insulin-like growth factor I binding [ISO]
- insulin-like growth factor II binding [ISO]
- insulin-like growth factor receptor binding [ISO]
- lipoic acid binding [ISO]
- phosphatidylinositol 3-kinase binding [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein domain specific binding [ISO]
- protein kinase activity [ISO]
- protein kinase binding [ISO]
- protein phosphatase binding [ISO]
- protein tyrosine kinase activity [ISO]
- receptor signaling protein tyrosine kinase activity [ISO]
Gene Ontology Cellular Component
SORBS1
Gene Ontology Biological Process
- cellular response to insulin stimulus [IC, IMP]
- focal adhesion assembly [IDA]
- glucose transport [IDA]
- insulin receptor signaling pathway [IC, IDA]
- positive regulation of establishment of protein localization to plasma membrane [IMP]
- positive regulation of glucose import [IMP]
- positive regulation of glycogen biosynthetic process [IMP]
- positive regulation of lipid biosynthetic process [IMP]
- positive regulation of signal transduction [IPI]
- stress fiber assembly [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cell-cell adherens junction [IDA]
- cell-substrate adherens junction [IDA]
- centrosome [ISO]
- cytoplasm [ISO]
- cytoskeleton [IDA]
- cytosol [IDA]
- flotillin complex [IC]
- focal adhesion [ISO]
- insulin receptor complex [ISO]
- membrane [IDA]
- membrane raft [IDA]
- nuclear matrix [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- stress fiber [IDA]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
A novel, multifuntional c-Cbl binding protein in insulin receptor signaling in 3T3-L1 adipocytes.
The protein product of the c-Cbl proto-oncogene is prominently tyrosine phosphorylated in response to insulin in 3T3-L1 adipocytes and not in 3T3-L1 fibroblasts. After insulin-dependent tyrosine phosphorylation, c-Cbl specifically associates with endogenous c-Crk and Fyn. These results suggest a role for tyrosine-phosphorylated c-Cbl in 3T3-L1 adipocyte activation by insulin. A yeast two-hybrid cDNA library prepared from fully differentiated 3T3-L1 adipocytes ... [more]
Throughput
- Low Throughput
Additional Notes
- figure 7.
Curated By
- BioGRID