An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Breast cancer metastasis suppressor 1 (BRMS1) is stabilized by the Hsp90 chaperone.

Hurst DR, Mehta A, Moore BP, Phadke PA, Meehan WJ, Accavitti MA, Shevde LA, Hopper JE, Xie Y, Welch DR, Samant RS

Breast cancer metastasis suppressor 1 (BRMS1) is a member of the mSin3-HDAC transcription co-repressor complex. However, the proteins associated with BRMS1 have not been fully identified. Yeast two-hybrid screen, immuno-affinity chromatography, and co-immunoprecipitation experiments were performed to identify BRMS1 interacting proteins (BIPs). In addition to known core mSin3 transcriptional complex components RBBP1 and mSDS3, BRMS1 interacted with other proteins including ... [more]

Biochem. Biophys. Res. Commun. Oct. 06, 2006; 348(4);1429-35 [Pubmed: 16919237]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

BRMS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NF-kappaB binding [IDA]protein binding [IPI]

Gene Ontology Cellular Component

HSPA4