BAIT

TAF1

TAF130, TAF145, KAT4, TafII145, TafII130, L000002748, YGR274C

TFIID subunit, involved in RNA pol II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression; relocalizes to the cytosol in response to hypoxia

GO Process (2)

GO Function (5)

GO Component (3)

Gene Ontology Biological Process

RNA polymerase II transcriptional preinitiation complex assembly [IMP]

transcription from RNA polymerase II promoter [IDA, IMP]

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
TBP-class protein binding [IDA]
chromatin binding [IDA]
histone acetyltransferase activity [IDA]
protein complex scaffold [IDA, IMP]

Gene Ontology Cellular Component

cytosol [IDA]

nucleus [IDA]

transcription factor TFIID complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

SGF29

YCL010C

Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation

GO Process (7)

GO Function (2)

GO Component (2)

Gene Ontology Biological Process

cellular protein complex localization [IMP]

cellular protein localization [IMP]

heterochromatin organization involved in chromatin silencing [IMP]

histone H3-K14 acetylation [IMP]

histone H3-K18 acetylation [IMP]

histone H3-K9 acetylation [IMP]

positive regulation of transcription from RNA polymerase II promoter [IMP]

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
methylated histone binding [IDA]

Gene Ontology Cellular Component

Ada2/Gcn5/Ada3 transcription activator complex [IMP, IPI]

SAGA complex [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Coiled-Coil Networking Shapes Cell Molecular Machinery.

Wang Y, Zhang X, Zhang H, Lu Y, Huang H, Dong X, Chen J, Dong J, Yang X, Hang H, Jiang T

The highly abundant alpha-helical coiled-coil motif not only mediates crucial protein-protein interactions in the cell, but is also an attractive scaffold in synthetic biology and material science and a potential target for disease intervention. Therefore, a systematic understanding of the coiled-coil interactions at the organismal level would help unravel the full spectrum of the biological function of this interaction motif ... [more]

Mol. Biol. Cell Aug. 08, 2012; 0(0); [Pubmed: 22875988]

Throughput

High Throughput

Additional Notes

Interaction between cloned coiled-coil domains

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TAF1 SGF29	Synthetic Lethality Synthetic Lethality A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.	van Pel DM (2013)	High	0.0147	BioGRID	821132

Curated By

BioGRID

Interaction Summary

TAF1

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]TBP-class protein binding [IDA]chromatin binding [IDA]histone acetyltransferase activity [IDA]protein complex scaffold [IDA, IMP]

Gene Ontology Cellular Component

SGF29

Gene Ontology Biological Process

Gene Ontology Molecular Function

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]methylated histone binding [IDA]

Gene Ontology Cellular Component

Two-hybrid

Publication

Coiled-Coil Networking Shapes Cell Molecular Machinery.

Throughput

Additional Notes

Related interactions

Curated By

RNA polymerase II core promoter sequence-specific DNA binding transcription factor activity involved in preinitiation complex assembly [IC]
TBP-class protein binding [IDA]
chromatin binding [IDA]
histone acetyltransferase activity [IDA]
protein complex scaffold [IDA, IMP]

RNA polymerase II transcription factor recruiting transcription factor activity [IMP]
methylated histone binding [IDA]