BAIT

IPI3

YNL182C
Component of the Rix1 complex and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene; other members include Rix1p, Ipi1p, and Ipi3p
Saccharomyces cerevisiae (S288c)
PREY

LHS1

CER1, SSI1, Hsp70 family chaperone LHS1, L000004102, YKL073W
Molecular chaperone of the endoplasmic reticulum lumen; involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway
GO Process (2)
GO Function (3)
GO Component (1)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Coiled-Coil Networking Shapes Cell Molecular Machinery.

Wang Y, Zhang X, Zhang H, Lu Y, Huang H, Dong X, Chen J, Dong J, Yang X, Hang H, Jiang T

The highly abundant alpha-helical coiled-coil motif not only mediates crucial protein-protein interactions in the cell, but is also an attractive scaffold in synthetic biology and material science and a potential target for disease intervention. Therefore, a systematic understanding of the coiled-coil interactions at the organismal level would help unravel the full spectrum of the biological function of this interaction motif ... [more]

Mol. Biol. Cell Aug. 08, 2012; 0(0); [Pubmed: 22875988]

Throughput

  • High Throughput

Additional Notes

  • Interaction between cloned coiled-coil domains

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
IPI3 LHS1
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-0.1776BioGRID
2010878

Curated By

  • BioGRID