BAIT

ARR1

ACR1, YAP8, L000004292, YPR199C

Transcriptional activator of the basic leucine zipper (bZIP) family; required for transcription of genes involved in resistance to arsenic compounds

GO Process (1)

GO Function (3)

GO Component (2)

Gene Ontology Biological Process

positive regulation of transcription from RNA polymerase II promoter in response to arsenic-containing substance [IGI, IMP]

Gene Ontology Molecular Function

DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]

Gene Ontology Cellular Component

cytoplasm [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

KEL1

L000003592, YHR158C

Protein required for proper cell fusion and cell morphology; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate; KEL1 has a paralog, KEL2, that arose from the whole genome duplication

GO Process (3)

GO Function (0)

GO Component (6)

Gene Ontology Biological Process

cytogamy [IGI, IMP, IPI]

negative regulation of exit from mitosis [IMP, IPI]

regulation of cell shape [IMP]

Gene Ontology Cellular Component

cell cortex [IDA]

cellular bud neck [IDA]

cellular bud tip [IDA]

cytoplasm [IDA]

mating projection tip [IDA]

prospore membrane [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Coiled-Coil Networking Shapes Cell Molecular Machinery.

Wang Y, Zhang X, Zhang H, Lu Y, Huang H, Dong X, Chen J, Dong J, Yang X, Hang H, Jiang T

The highly abundant alpha-helical coiled-coil motif not only mediates crucial protein-protein interactions in the cell, but is also an attractive scaffold in synthetic biology and material science and a potential target for disease intervention. Therefore, a systematic understanding of the coiled-coil interactions at the organismal level would help unravel the full spectrum of the biological function of this interaction motif ... [more]

Mol. Biol. Cell Aug. 08, 2012; 0(0); [Pubmed: 22875988]

Throughput

High Throughput

Additional Notes

Interaction between cloned coiled-coil domains

Curated By

BioGRID

Interaction Summary

ARR1

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [IDA]RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]

Gene Ontology Cellular Component

KEL1

Gene Ontology Biological Process

Gene Ontology Cellular Component

Two-hybrid

Publication

Coiled-Coil Networking Shapes Cell Molecular Machinery.

Throughput

Additional Notes

Curated By

DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]