Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A systematic analysis of human CHMP protein interactions: additional MIT domain-containing proteins bind to multiple components of the human ESCRT III complex.

Tsang HT, Connell JW, Brown SE, Thompson A, Reid E, Sanderson CM

In Saccharomyces cerevisiae 6 closely related proteins (Did2p, Vps2p, Vps24p, Vps32p, Vps60p, Vps20p) form part of the extended ESCRT III complex. This complex is required for the formation of multivesicular bodies and the degradation of internalized transmembrane receptor proteins. In contrast the human genome encodes 10 homologous proteins (CHMP1A (approved gene symbol PCOLN3), 1B, 2A, 2B, 3 (approved gene symbol ... [more]

Genomics Sep. 01, 2006; 88(3);333-46 [Pubmed: 16730941]

Throughput

High Throughput

Additional Notes

table 1.

Curated By

BioGRID

Interaction Summary

CHMP2B

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein domain specific binding [IPI]

Gene Ontology Cellular Component

MALSU1