Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Yeast two-hybrid identification of prostatic proteins interacting with human sex hormone-binding globulin.

Pope SN, Lee IR

Yeast two-hybrid (Y2H) screening of a prostate cDNA library with the cDNA for sex hormone-binding globulin (SHBG) has been used to identify proteins through which SHBG may exert autocrine or paracrine effects on sex steroid target tissues. The library screen gave 230 positive interactions of which around 60 have been sequenced. Of the proteins identified to date from database (BLAST) ... [more]

J. Steroid Biochem. Mol. Biol. Feb. 01, 2005; 94(1);203-8 [Pubmed: 15862967]

Throughput

  • Low Throughput

Curated By

  • BioGRID