BAIT

ZBTB33

ZNF-kaiso, ZNF348
zinc finger and BTB domain containing 33
GO Process (2)
GO Function (3)
GO Component (5)
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The human enhancer blocker CTC-binding factor interacts with the transcription factor Kaiso.

Defossez PA, Kelly KF, Filion GJ, Perez-Torrado R, Magdinier F, Menoni H, Nordgaard CL, Daniel JM, Gilson E

CTC-binding factor (CTCF) is a DNA-binding protein of vertebrates that plays essential roles in regulating genome activity through its capacity to act as an enhancer blocker. We performed a yeast two-hybrid screen to identify protein partners of CTCF that could regulate its activity. Using full-length CTCF as bait we recovered Kaiso, a POZ-zinc finger transcription factor, as a specific binding ... [more]

J. Biol. Chem. Dec. 30, 2005; 280(52);43017-23 [Pubmed: 16230345]

Throughput

  • Low Throughput

Curated By

  • BioGRID