CRKL
Gene Ontology Biological Process
Gene Ontology Molecular Function
STAT5A
Gene Ontology Biological Process
- 2-oxoglutarate metabolic process [ISS]
- JAK-STAT cascade [TAS]
- JAK-STAT cascade involved in growth hormone signaling pathway [TAS]
- allantoin metabolic process [ISS]
- citrate metabolic process [ISS]
- creatine metabolic process [ISS]
- creatinine metabolic process [ISS]
- fatty acid metabolic process [ISS]
- isoleucine metabolic process [ISS]
- oxaloacetate metabolic process [ISS]
- prolactin signaling pathway [ISS]
- regulation of multicellular organism growth [ISS]
- regulation of transcription from RNA polymerase II promoter [TAS]
- succinate metabolic process [ISS]
- taurine metabolic process [ISS]
- valine metabolic process [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- cytosol [TAS]
- nucleoplasm [TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Erythropoietin induces tyrosine phosphorylation of Jak2, STAT5A, and STAT5B in primary cultured human erythroid precursors.
We examined signaling by erythropoietin in highly purified human colony forming unit-erythroid cells, generated in vitro from CD34(+) cells. We found that erythropoietin induces tyrosine phosphorylation of Jak2, STAT5A, and STAT5B. Tyrosine phosphorylation of Jak2 reaches a peak around 10 minutes after stimulation and is maximum at 5 U/mL of erythropoietin. Tyrosine phosphorylation of STAT5 is accompanied by the translocation ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CRKL STAT5A | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| CRKL STAT5A | Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro. | Low | - | BioGRID | - |
Curated By
- BioGRID