BAIT

MYOC

GLC1A, GPOA, JOAG, JOAG1, TIGR, myocilin
myocilin, trabecular meshwork inducible glucocorticoid response
Homo sapiens
PREY

EEF1A1

CCS-3, CCS3, EE1A1, EEF-1, EEF1A, EF-Tu, EF1A, GRAF-1EF, HNGC:16303, LENG7, PTI1, eEF1A-1, RP11-505P4.2
eukaryotic translation elongation factor 1 alpha 1
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The identification of myocilin-associated proteins in the human trabecular meshwork.

Fautsch MP, Vrabel AM, Johnson DH

Myocilin forms high molecular weight complexes in vivo presumably due to interaction with itself and other myocilin binding proteins. To identify myocilin interacting proteins, yeast 2-hybrid analysis was performed on >1x10(6) human trabecular meshwork cDNA clones. Coimmunoprecipitation and Far Western analysis were also performed on cell lysates obtained from fresh human trabecular meshworks or cultured human monolayer trabecular cell lines. ... [more]

Exp. Eye Res. Jun. 01, 2006; 82(6);1046-52 [Pubmed: 16289162]

Throughput

  • Low Throughput

Curated By

  • BioGRID