Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Two N-terminal domains of Kv4 K(+) channels regulate binding to and modulation by KChIP1.

Scannevin RH, Wang K, Jow F, Megules J, Kopsco DC, Edris W, Carroll KC, Lue Q, Xu W, Xu Z, Katz AH, Olland S, Lin L, Taylor M, Stahl M, Malakian K, Somers W, Mosyak L, Bowlby MR, Chanda P, Rhodes KJ

The family of calcium binding proteins called KChIPs associates with Kv4 family K(+) channels and modulates their biophysical properties. Here, using mutagenesis and X-ray crystallography, we explore the interaction between Kv4 subunits and KChIP1. Two regions in the Kv4.2 N terminus, residues 7-11 and 71-90, are necessary for KChIP1 modulation and interaction with Kv4.2. When inserted into the Kv1.2 N ... [more]

Neuron Feb. 19, 2004; 41(4);587-98 [Pubmed: 14980207]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
KCNIP1 KCND2	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	An WF (2000)	Low	-	BioGRID	-
KCND2 KCNIP1	Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.	An WF (2000)	Low	-	BioGRID	-
KCND2 KCNIP1	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Lin YL (2004)	Low	-	BioGRID	-
KCNIP1 KCND2	Reconstituted Complex Reconstituted Complex An interaction is detected between purified proteins in vitro.	Lin YL (2004)	Low	-	BioGRID	-
KCND2 KCNIP1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Scannevin RH (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

KCND2

Gene Ontology Biological Process

Gene Ontology Molecular Function

A-type (transient outward) potassium channel activity [IBA]delayed rectifier potassium channel activity [IBA]protein binding [IPI]voltage-gated potassium channel activity [NAS]

Gene Ontology Cellular Component

KCNIP1