An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

E2FBP1/hDril1 modulates cell growth through downregulation of promyelocytic leukemia bodies.

Fukuyo Y, Mogi K, Tsunematsu Y, Nakajima T

Promyelocytic leukemia nuclear bodies (PML-NBs) comprise multiple regulatory factors and play crucial roles in the maintenance of cellular integrity, while unregulated activation of PML-NBs induces death and premature senescence. Hence, the function of PML-NBs must be directed properly; however, the mechanism that regulates PML-NBs remains unclear. In this paper, we show that PML-NBs are disintegrated by an AT-rich interaction domain ... [more]

Cell Death Differ. Jul. 01, 2004; 11(7);747-59 [Pubmed: 15017387]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
ARID3A UBE2E3	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Fukuyo Y (2004)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

ARID3A

Gene Ontology Molecular Function

protein binding [IPI]

Gene Ontology Cellular Component

UBE2E3

Gene Ontology Biological Process