FOS
Gene Ontology Biological Process
- SMAD protein signal transduction [ISO]
- cellular response to calcium ion [IDA]
- cellular response to extracellular stimulus [IMP]
- cellular response to reactive oxygen species [ISO]
- nervous system development [IMP]
- positive regulation of osteoclast differentiation [IDA]
- positive regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- positive regulation of transcription, DNA-templated [ISO]
- regulation of transcription, DNA-templated [IDA, IMP]
- response to drug [IDA]
- response to muscle stretch [IDA]
- skeletal muscle cell differentiation [IMP]
- transcription from RNA polymerase II promoter [IMP, ISO]
- transforming growth factor beta receptor signaling pathway [ISO]
Gene Ontology Molecular Function- DNA binding [IDA, ISO]
- R-SMAD binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- double-stranded DNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA, IMP, ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- DNA binding [IDA, ISO]
- R-SMAD binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [IDA]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IMP]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- chromatin binding [IDA]
- double-stranded DNA binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- sequence-specific DNA binding [ISO]
- sequence-specific DNA binding transcription factor activity [IDA, IMP, ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
Gene Ontology Cellular Component
EEF1D
Gene Ontology Biological Process
Gene Ontology Molecular Function
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Cell-free cotranslation and selection using in vitro virus for high-throughput analysis of protein-protein interactions and complexes.
We have developed a simple and totally in vitro selection procedure based on cell-free cotranslation using a highly stable and efficient in vitro virus (IVV). Cell-free cotranslation of tagged bait and prey proteins is advantageous for the formation of protein complexes and allows high-throughput analysis of protein-protein interactions (PPI) as a result of providing in vitro instead of in vivo ... [more]
Throughput
- Low Throughput
Additional Notes
- Cell-free cotranslation of tagged bait and prey proteins in an in vitro virus.
Curated By
- BioGRID