PREY

ARRB2

ARB2, ARR2, BARR2
arrestin, beta 2
Homo sapiens

FRET

An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.

Publication

Temporal profiling of orexin receptor-arrestin-ubiquitin complexes reveals differences between receptor subtypes.

Dalrymple MB, Jaeger WC, Eidne KA, Pfleger KD

Orexin G protein-coupled receptors (OxRs) and their cognate agonists have been implicated in a number of disorders since their recent discovery, ranging from narcolepsy to formation of addictive behavior. Bioluminescence resonance energy transfer assays of agonist-occupied OxRs provided evidence for a strong dose-dependent interaction with both trafficking proteins β-arrestin 1 and 2 that required unusually high agonist concentrations compared with ... [more]

J. Biol. Chem. May. 13, 2011; 286(19);16726-33 [Pubmed: 21378163]

Throughput

  • Low Throughput

Curated By

  • BioGRID