An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Specific in vivo binding of activator of G protein signalling 1 to the Gbeta1 subunit.

Hiskens R, Vatish M, Hill C, Davey J, Ladds G

Activator of G protein signalling 1 (AGS1) is a Ras-like protein that affects signalling through heterotrimeric G proteins. Previous in vitro studies suggest that AGS1 can bind to G(alpha)-GDP subunits and promote nucleotide exchange, leading to activation of intracellular signalling pathways. This model is consistent with in vivo evidence demonstrating that AGS1 activates both G(alpha)- and G(betagamma)-dependent pathways in the ... [more]

Biochem. Biophys. Res. Commun. Dec. 02, 2005; 337(4);1038-46 [Pubmed: 16225846]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
RASD1 GNB1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Hiskens R (2005)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

GNB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activity [IDA]GTPase binding [IPI]protein binding [IPI]protein complex binding [IDA]

Gene Ontology Cellular Component

RASD1