BAIT

IRAK1

IRAK, pelle
interleukin-1 receptor-associated kinase 1
GO Process (37)
GO Function (8)
GO Component (7)

Gene Ontology Biological Process

Homo sapiens
PREY

NLRP12

CLR19.3, FCAS2, NALP12, PAN6, PYPAF7, RNO, RNO2
NLR family, pyrin domain containing 12
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The CATERPILLER protein monarch-1 is an antagonist of toll-like receptor-, tumor necrosis factor alpha-, and Mycobacterium tuberculosis-induced pro-inflammatory signals.

Williams KL, Lich JD, Duncan JA, Reed W, Rallabhandi P, Moore C, Kurtz S, Coffield VM, Accavitti-Loper MA, Su L, Vogel SN, Braunstein M, Ting JP

The CATERPILLER (CLR, also NOD and NLR) proteins share structural similarities with the nucleotide binding domain (NBD)-leucine-rich repeat (LRR) superfamily of plant disease-resistance (R) proteins and are emerging as important immune regulators in animals. CLR proteins contain NBD-LRR motifs and are linked to a limited number of distinct N-terminal domains including transactivation, CARD (caspase activation and recruitment), and pyrin domains ... [more]

J. Biol. Chem. Dec. 02, 2005; 280(48);39914-24 [Pubmed: 16203735]

Throughput

  • Low Throughput

Curated By

  • BioGRID