An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The expression and the regulatory role of OX40 and 4-1BB heterodimer in activated human T cells.

Ma BY, Mikolajczak SA, Danesh A, Hosiawa KA, Cameron CM, Takaori-Kondo A, Uchiyama T, Kelvin DJ, Ochi A

OX40 and 4-1BB are members of the tumor necrosis factor (TNF) family of costimulatory receptors whose signaling is important for differential immune responses mediated by CD4+ or CD8+ T cells. Although activated T cells may acquire OX40/4-1BB double-positive phenotype and signaling from each receptor is expected to influence cell functions, the relevance between OX40 and 4-1BB has never been investigated ... [more]

Blood Sep. 15, 2005; 106(6);2002-10 [Pubmed: 15941918]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

TRAF4

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]WW domain binding [IPI]protein binding [IPI]thioesterase binding [IPI]tumor necrosis factor receptor binding [IPI]ubiquitin protein ligase binding [IPI]

Gene Ontology Cellular Component

TNFRSF4