Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Human follitropin receptor (FSHR) interacts with the adapter protein 14-3-3tau.

Cohen BD, Nechamen CA, Dias JA

The human follitropin (follicle stimulating hormone, FSH) receptor (FSHR) is a G protein-coupled receptor (GPCR). To identify cytoplasmic proteins that may regulate FSHR function, a yeast-based interaction trap was performed. A linked construct of the first and second intracellular loops (iL1-iL2 bait) of FSHR was used as bait and a human ovarian cDNA library was used as prey. Among the ... [more]

Mol. Cell. Endocrinol. May. 31, 2004; 220(1);1-7 [Pubmed: 15196694]

Throughput

  • Low Throughput

Curated By

  • BioGRID