PKN1
Gene Ontology Biological Process
Gene Ontology Molecular Function- GTP-Rho binding [IDA]
- Rac GTPase binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- histone binding [IDA]
- histone deacetylase binding [IDA]
- histone kinase activity (H3-T11 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA, IMP]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA]
- GTP-Rho binding [IDA]
- Rac GTPase binding [IDA]
- androgen receptor binding [IDA]
- chromatin binding [IDA]
- histone binding [IDA]
- histone deacetylase binding [IDA]
- histone kinase activity (H3-T11 specific) [IDA]
- ligand-dependent nuclear receptor transcription coactivator activity [IDA, IMP]
- protein binding [IPI]
- protein kinase C binding [IPI]
- protein kinase activity [TAS]
- protein serine/threonine kinase activity [IDA]
TRAF2
Gene Ontology Biological Process
- activation of NF-kappaB-inducing kinase activity [IMP]
- activation of cysteine-type endopeptidase activity involved in apoptotic process [TAS]
- apoptotic process [TAS]
- apoptotic signaling pathway [TAS]
- cellular protein complex assembly [ISS]
- innate immune response [TAS]
- negative regulation of neuron death [TAS]
- positive regulation of JUN kinase activity [IDA]
- positive regulation of NF-kappaB transcription factor activity [IDA, IMP]
- positive regulation of T cell activation [IC]
- positive regulation of T cell cytokine production [IMP]
- positive regulation of extrinsic apoptotic signaling pathway [IMP]
- positive regulation of interleukin-2 production [IMP]
- positive regulation of protein homodimerization activity [IMP]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA, TAS]
- protein complex assembly [TAS]
- protein homotrimerization [IPI]
- regulation of apoptotic process [IDA]
- regulation of extrinsic apoptotic signaling pathway in absence of ligand [TAS]
- signal transduction [TAS]
- tumor necrosis factor-mediated signaling pathway [IDA]
Gene Ontology Molecular Function- CD40 receptor binding [ISS]
- enzyme binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein phosphatase binding [IPI]
- signal transducer activity [NAS]
- sphingolipid binding [IDA]
- thioesterase binding [IPI]
- tumor necrosis factor receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA]
- CD40 receptor binding [ISS]
- enzyme binding [IPI]
- identical protein binding [IPI]
- protein binding [IPI]
- protein phosphatase binding [IPI]
- signal transducer activity [NAS]
- sphingolipid binding [IDA]
- thioesterase binding [IPI]
- tumor necrosis factor receptor binding [IPI]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA]
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Protein kinase PKN1 associates with TRAF2 and is involved in TRAF2-NF-kappaB signaling pathway.
PKN1 is a fatty acid and Rho-activated serine/threonine protein kinase whose catalytic domain is highly homologous to protein kinase C (PKC) family. In yeast two-hybrid screening for PKN1 binding proteins, we identified tumor necrosis factor alpha (TNFalpha) receptor-associated factor 2 (TRAF2). TRAF2 is one of the major mediators of TNF receptor superfamily transducing TNF signal to various functional targets, including ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PKN1 TRAF2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PKN1 TRAF2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low/High | - | BioGRID | - | |
| PKN1 TRAF2 | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | High | - | BioGRID | 2719075 |
Curated By
- BioGRID