GNB1
Gene Ontology Biological Process
- G-protein coupled receptor signaling pathway [IDA, ISO, TAS]
- GTP catabolic process [ISA, ISO]
- cardiac muscle cell apoptotic process [ISO]
- cell proliferation [IDA]
- cellular response to hypoxia [ISO]
- phospholipase C-activating G-protein coupled receptor signaling pathway [IDA]
- phototransduction, visible light [ISA]
- positive regulation of cytosolic calcium ion concentration [ISO]
- retina development in camera-type eye [IMP]
- sensory perception of taste [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
DYNC1I1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The betagamma subunit of heterotrimeric G proteins interacts with RACK1 and two other WD repeat proteins.
A yeast two-hybrid approach was used to discern possible new effectors for the betagamma subunit of heterotrimeric G proteins. Three of the clones isolated are structurally similar to Gbeta, each exhibiting the WD40 repeat motif. Two of these proteins, the receptor for activated C kinase 1 (RACK1) and the dynein intermediate chain, co-immunoprecipitate with Gbetagamma using an anti-Gbeta antibody. The ... [more]
Throughput
- High Throughput
Curated By
- BioGRID