CRKL
Gene Ontology Molecular Function
PXN
Gene Ontology Biological Process
- activation of MAPK activity [ISO]
- branching morphogenesis of an epithelial tube [ISO]
- cellular component movement [ISO]
- cellular response to reactive oxygen species [ISO]
- cytoskeleton organization [ISO]
- focal adhesion assembly [ISO]
- growth hormone receptor signaling pathway [ISO]
- integrin-mediated signaling pathway [ISO]
- lamellipodium assembly [ISO]
- peptidyl-tyrosine phosphorylation [ISO]
- positive regulation of protein kinase activity [ISO]
- regulation of cell shape [ISO]
- substrate adhesion-dependent cell spreading [ISO]
- transforming growth factor beta receptor signaling pathway [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Structural requirements for function of the Crkl adapter protein in fibroblasts and hematopoietic cells.
Crkl is an adapter protein and phosphotyrosine-containing substrate implicated in transformation by the bcr-abl oncogene and in signaling by cytokines. When phosphorylated, Crkl binds through its Src homology 2 (SH2) domain to other tyrosine phosphoproteins such as paxillin and Cbl. Overexpression of Crkl in fibroblasts induces transformation. Here we examine the role of Crkl in hematopoietic cells and find that ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID