Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

The cationic region of Rhes mediates its interactions with specific Gbeta subunits.

Hill C, Goddard A, Ladds G, Davey J

Ras homologue enriched in striatum (Rhes) is a small monomeric G protein which functions in a variety of cellular processes, including attenuation of G protein-coupled receptor (GPCR) signalling. There have been many studies into the effects of Rhes, but there is no molecular information about how Rhes might bring about these effects. Rhes shares striking sequence homology to AGS1 (activator ... [more]

Cell. Physiol. Biochem. Mar. 04, 2009; 23(1);1-8 [Pubmed: 19255495]

Throughput

  • Low Throughput

Curated By

  • BioGRID