Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Characterization of Mesenchyme Homeobox 2 (MEOX2) transcription factor binding to RING finger protein 10.

Lin J, Friesen MT, Bocangel P, Cheung D, Rawszer K, Wigle JT

The molecular mechanisms by which Mesenchyme Homeobox 2 (Meox2) regulates the proliferation, differentiation and migration of vascular smooth muscle cells and cardiomyocytes are not known. The discovery of MEOX2 binding proteins will aid in understanding how MEOX2 functions as a regulator of these key cellular processes. To identify MEOX2 binding proteins, a yeast two-hybrid screen of a human heart cDNA ... [more]

Mol. Cell. Biochem. Jul. 01, 2005; 275(1);75-84 [Pubmed: 16335786]

Throughput

  • Low Throughput

Curated By

  • BioGRID