An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Kv8.1, a new neuronal potassium channel subunit with specific inhibitory properties towards Shab and Shaw channels.

Hugnot JP, Salinas M, Lesage F, Guillemare E, de Weille J, Heurteaux C, Mattei MG, Lazdunski M

Outward rectifier K+ channels have a characteristic structure with six transmembrane segments and one pore region. A new member of this family of transmembrane proteins has been cloned and called Kv8.1. Kv8.1 is essentially present in the brain where it is located mainly in layers II, IV and VI of the cerebral cortex, in hippocampus, in CA1-CA4 pyramidal cell layer ... [more]

EMBO J. Jul. 01, 1996; 15(13);3322-31 [Pubmed: 8670833]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

KCNV1

Gene Ontology Biological Process

Gene Ontology Molecular Function

delayed rectifier potassium channel activity [IBA]ion channel inhibitor activity [TAS]potassium channel regulator activity [TAS]

Gene Ontology Cellular Component

KCNB2