BAIT

KPNA2

IPOA1, QIP2, RCH1, SRP1alpha
karyopherin alpha 2 (RAG cohort 1, importin alpha 1)
Homo sapiens
PREY

ZBTB33

AW260253, E130014G12Rik, Kaiso, Znf-kaiso, RP23-322E15.9
zinc finger and BTB domain containing 33
GO Process (2)
GO Function (3)
GO Component (5)
Mus musculus

Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Nuclear import of the BTB/POZ transcriptional regulator Kaiso.

Kelly KF, Otchere AA, Graham M, Daniel JM

Kaiso is a BTB/POZ transcription factor that functions in vitro as a transcriptional repressor of the matrix metalloproteinase gene matrilysin and the non-canonical Wnt signaling gene Wnt-11, and as an activator of the acetylcholine-receptor-clustering gene rapsyn. Similar to other BTB/POZ proteins (e.g. Bcl-6, PLZF, HIC-1), endogenous Kaiso localizes predominantly to the nuclei of mammalian cells. To date, however, the mechanism ... [more]

J. Cell. Sci. Dec. 01, 2004; 117(0);6143-52 [Pubmed: 15564377]

Throughput

  • Low Throughput

Additional Notes

  • Source of Importin alpha 2 not clear

Curated By

  • BioGRID