Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

Identification of proteins that interact with the central coiled-coil region of the human protein kinase NEK1.

Surpili MJ, Delben TM, Kobarg J

NEK protein kinases are evolutionarily conserved kinases structurally related to the Aspergillus nidulans mitotic regulator NIMA. At least nine members of the NEK family in vertebrates have been described to date, but for most of them the interacting protein partners are unknown. The pleiotropic deleterious effects and the formation of kidney cysts caused by NEK1 mutation in mice emphasize its ... [more]

Biochemistry Dec. 30, 2003; 42(51);15369-76 [Pubmed: 14690447]

Throughput

  • Low Throughput

Curated By

  • BioGRID