An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

A sperm component, HSD-3.8 (SPAG1), interacts with G-protein beta 1 subunit and activates extracellular signal-regulated kinases (ERK).

Liu N, Qiao Y, Cai C, Lin W, Zhang J, Miao S, Zong S, Koide SS, Wang L

HSD-3.8 cDNA (accession number AF311312) encodes a human sperm component. A 0.7 kb fragment (HSD-0.7) containing three immunological epitopes of HSD-3.8 cDNA was prepared and expressed in E. coli. Immunization of female rats with the recombinant HSD-0.7 proteins induced infertility. A cDNA fragment encoding the C-terminal 144 amino acids of human G-protein beta l subunit (Gbeta1-C144) was screened by yeast ... [more]

Front. Biosci. Dec. 22, 2005; 11(0);1679-89 [Pubmed: 16368546]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
SPAG1 GNB1	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Liu N (2005)	High	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

SPAG1

Gene Ontology Biological Process

Gene Ontology Cellular Component

GNB1

Gene Ontology Biological Process

Gene Ontology Molecular Function

GTPase activity [IDA]GTPase binding [IPI]protein binding [IPI]protein complex binding [IDA]