Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.

Lim J, Hao T, Shaw C, Patel AJ, Szabo G, Rual JF, Fisk CJ, Li N, Smolyar A, Hill DE, Barabasi AL, Vidal M, Zoghbi HY

Many human inherited neurodegenerative disorders are characterized by loss of balance due to cerebellar Purkinje cell (PC) degeneration. Although the disease-causing mutations have been identified for a number of these disorders, the normal functions of the proteins involved remain, in many cases, unknown. To gain insight into the function of proteins involved in PC degeneration, we developed an interaction network ... [more]

Cell May. 19, 2006; 125(4);801-14 [Pubmed: 16713569]

Throughput

High Throughput

Curated By

BioGRID

Interaction Summary

ATXN1

Gene Ontology Biological Process

Gene Ontology Molecular Function

identical protein binding [IPI]poly(G) binding [IDA]poly(U) RNA binding [IDA]protein C-terminus binding [IPI]protein binding [IPI]protein self-association [IDA]

Gene Ontology Cellular Component

WNK1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATP binding [IDA]chloride channel inhibitor activity [IDA]phosphatase binding [IDA]protein binding [IPI]protein serine/threonine kinase activity [IDA, TAS]

Gene Ontology Cellular Component

Two-hybrid

Publication

A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration.

Throughput

Curated By

identical protein binding [IPI]
poly(G) binding [IDA]
poly(U) RNA binding [IDA]
protein C-terminus binding [IPI]
protein binding [IPI]
protein self-association [IDA]

ATP binding [IDA]
chloride channel inhibitor activity [IDA]
phosphatase binding [IDA]
protein binding [IPI]
protein serine/threonine kinase activity [IDA, TAS]