CRKL
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
SOS1
Gene Ontology Biological Process
- B cell homeostasis [IGI]
- Ras protein signal transduction [ISO]
- fibroblast growth factor receptor signaling pathway [IGI]
- lymphocyte homeostasis [IGI]
- neurotrophin TRK receptor signaling pathway [ISO]
- positive regulation of Ras GTPase activity [IMP, ISO]
- positive regulation of small GTPase mediated signal transduction [ISO]
- regulation of T cell differentiation in thymus [IGI]
- regulation of T cell proliferation [IGI]
- regulation of pro-B cell differentiation [IGI]
- small GTPase mediated signal transduction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Direct binding of CRKL to BCR-ABL is not required for BCR-ABL transformation.
CRKL has previously been shown to be a major tyrosine phosphorylated protein in neutrophils of patients with BCR-ABL+ chronic myelogenous leukemia and in cell lines expressing BCR-ABL CRKL and BCR-ABL form a complex as demonstrated by coimmunoprecipitation and are capable of a direct interaction in a yeast two-hybrid assay. We have mapped the site of interaction of CRKL and BCR-ABL ... [more]
Throughput
- Low Throughput
Related interactions
Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
---|---|---|---|---|---|---|
CRKL SOS1 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.7725 | BioGRID | 2675676 |
Curated By
- BioGRID