WT1
Gene Ontology Biological Process
- RNA splicing [ISS]
- adrenal cortex formation [ISS]
- adrenal gland development [IGI]
- branching involved in ureteric bud morphogenesis [IGI]
- camera-type eye development [ISS]
- cardiac muscle cell fate commitment [ISS]
- cellular response to cAMP [IEP]
- cellular response to gonadotropin stimulus [IDA]
- diaphragm development [ISS]
- epithelial cell differentiation [ISS]
- germ cell development [ISS]
- glomerular basement membrane development [IMP]
- glomerular visceral epithelial cell differentiation [IBA, ISS]
- glomerulus development [IGI]
- gonad development [ISS]
- heart development [IGI]
- kidney development [IGI]
- male genitalia development [ISS]
- male gonad development [IEP]
- mesenchymal to epithelial transition [ISS]
- metanephric S-shaped body morphogenesis [IGI]
- metanephric epithelium development [IEP]
- metanephric mesenchyme development [ISS]
- negative regulation of apoptotic process [IGI]
- negative regulation of cell growth [IDA]
- negative regulation of cell proliferation [IDA]
- negative regulation of female gonad development [ISS]
- negative regulation of metanephric glomerular mesangial cell proliferation [ISS]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- negative regulation of translation [IDA]
- positive regulation of apoptotic process [IDA]
- positive regulation of heart growth [ISS]
- positive regulation of male gonad development [ISS]
- positive regulation of metanephric ureteric bud development [ISS]
- positive regulation of transcription from RNA polymerase II promoter [ISS]
- positive regulation of transcription, DNA-templated [IDA, IGI]
- posterior mesonephric tubule development [ISS]
- regulation of organ formation [ISS]
- regulation of transcription from RNA polymerase II promoter [ISS]
- regulation of transcription, DNA-templated [ISS, NAS]
- sex determination [IDA]
- thorax and anterior abdomen determination [ISS]
- tissue development [ISS]
- ureteric bud development [ISS]
- vasculogenesis [ISS]
- visceral serous pericardium development [IGI]
Gene Ontology Molecular Function- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [IDA]
- C2H2 zinc finger domain binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISS]
- protein binding [IPI]
- sequence-specific DNA binding [IDA]
- sequence-specific DNA binding transcription factor activity [ISS, NAS]
- transcription regulatory region DNA binding [IDA]
- zinc ion binding [IDA]
Gene Ontology Cellular Component
- cytoplasm [IBA, ISS]
- nuclear speck [IDA]
- nucleoplasm [IDA]
- nucleus [IDA]
HSPA4
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Inhibition of cellular proliferation by the Wilms tumor suppressor WT1 requires association with the inducible chaperone Hsp70.
The Wilms tumor suppressor WT1 encodes a zinc finger transcription factor that is expressed in glomerular podocytes during a narrow window in kidney development. By immunoprecipitation and protein microsequencing analysis, we have identified a major cellular protein associated with endogenous WT1 to be the inducible chaperone Hsp70. WT1 and Hsp70 are physically associated in embryonic rat kidney cells, in primary ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID