BAIT
MRGPRX1
GPCR, MGRG2, MRGX1, SNSR4
MAS-related GPR, member X1
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY
GNG2
guanine nucleotide binding protein (G protein), gamma 2
GO Process (9)
GO Function (1)
GO Component (4)
Gene Ontology Biological Process
- adenylate cyclase-activating dopamine receptor signaling pathway [ISS]
- blood coagulation [TAS]
- cellular response to catecholamine stimulus [ISS]
- cellular response to glucagon stimulus [TAS]
- cellular response to prostaglandin E stimulus [ISS]
- energy reserve metabolic process [TAS]
- platelet activation [TAS]
- small molecule metabolic process [TAS]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
FRET
An interaction is inferred when close proximity of interaction partners is detected by fluorescence resonance energy transfer between pairs of fluorophore-labeled molecules, such as occurs between CFP (donor) and YFP (acceptor) fusion proteins.
Publication
Real-time monitoring of receptor and G-protein interactions in living cells.
G protein-coupled receptors (GPCRs) represent the largest family of proteins involved in signal transduction. Here we present a bioluminescence resonance energy transfer (BRET) assay that directly monitors in real time the early interactions between human GPCRs and their cognate G-protein subunits in living human cells. In addition to detecting basal precoupling of the receptors to Galpha-, Gbeta- and Ggamma-subunits, BRET ... [more]
Nat. Methods Mar. 01, 2005; 2(3);177-84 [Pubmed: 15782186]
Throughput
- Low Throughput
Additional Notes
- interaction detected by BRET
Curated By
- BioGRID