NCK1
Gene Ontology Biological Process
- T cell activation [ISO]
- actin filament organization [IMP]
- cell migration [IMP]
- lamellipodium assembly [IMP]
- negative regulation of cell death [ISO]
- negative regulation of protein kinase activity [ISO]
- positive regulation of T cell proliferation [ISO]
- positive regulation of actin filament polymerization [IGI, ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- response to other organism [IMP]
- substrate-dependent cell migration, cell extension [IGI]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TNK2
Gene Ontology Biological Process
- cell differentiation [IBA]
- cell migration [IBA]
- innate immune response [IBA]
- peptidyl-tyrosine autophosphorylation [IBA]
- phosphorylation [ISO]
- positive regulation of peptidyl-tyrosine phosphorylation [ISO]
- regulation of cell proliferation [IBA]
- regulation of clathrin-mediated endocytosis [ISO]
- transmembrane receptor protein tyrosine kinase signaling pathway [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Activation of the nonreceptor protein tyrosine kinase Ack by multiple extracellular stimuli.
Ack/Ack1 is a nonreceptor protein tyrosine kinase that comprises a tyrosine kinase core, an SH3 domain, a Cdc42-binding region, a Ralt homology region, and a proline-rich region. Here we describe a detailed characterization of the Ack protein as well as the chromosomal localization of human Ack (chromosome 3q29) and the primary structure of murine Ack. We demonstrate that Ack is ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| TNK2 NCK1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID