An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Analysis of articulation between clathrin and retromer in retrograde sorting on early endosomes.

Popoff V, Mardones GA, Bai SK, Chambon V, Tenza D, Burgos PV, Shi A, Benaroch P, Urbe S, Lamaze C, Grant BD, Raposo G, Johannes L

Clathrin and retromer have key functions for retrograde trafficking between early endosomes and the trans-Golgi network (TGN). Previous studies on Shiga toxin suggested that these two coat complexes operate in a sequential manner. Here, we show that the curvature recognition subunit component sorting nexin 1 (SNX1) of retromer interacts with receptor-mediated endocytosis-8 (RME-8) protein, and that RME-8 and SNX1 colocalize ... [more]

Traffic Dec. 01, 2009; 10(12);1868-80 [Pubmed: 19874558]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SNX1

Gene Ontology Biological Process

Gene Ontology Molecular Function

epidermal growth factor receptor binding [IDA]insulin receptor binding [IDA]leptin receptor binding [IDA]phosphatidylinositol binding [IDA]protein binding [IPI]transferrin receptor binding [IDA]

Gene Ontology Cellular Component

DNAJC13