BAIT

KLHL24

DRE1, KRIP6
kelch-like family member 24
GO Process (0)
GO Function (0)
GO Component (0)
Homo sapiens
PREY

PICK1

PICK, PRKCABP, RP5-1039K5.11-004
protein interacting with PRKCA 1
Homo sapiens

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

The BTB/kelch protein, KRIP6, modulates the interaction of PICK1 with GluR6 kainate receptors.

Laezza F, Wilding TJ, Sequeira S, Craig AM, Huettner JE

Neuronal proteins of the BTB/kelch and PDZ domain families interact with different regions of the cytoplasmic C-terminal domain of the GluR6 kainate receptor subunit. The BTB/kelch protein KRIP6 binds within a 58 amino acid segment of GluR6 proximal to the plasma membrane. In contrast, PDZ domain proteins, such as PICK1 and PSD95, interact with the last 4 residues of the ... [more]

Neuropharmacology Dec. 01, 2008; 55(7);1131-9 [Pubmed: 18692513]

Throughput

  • Low Throughput

Curated By

  • BioGRID