BAIT

VIPAS39

C14orf133, SPE-39, SPE39, VIPAR, VPS16B, hSPE-39
VPS33B interacting protein, apical-basolateral polarity regulator, spe-39 homolog
GO Process (2)
GO Function (1)
GO Component (5)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens
PREY

VPS39

A230065P22Rik, AW743070, Vam6, Vam6P, mVam6, RP23-348L16.12-003
vacuolar protein sorting 39 (yeast)
GO Process (1)
GO Function (1)
GO Component (2)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Mus musculus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SPE-39 family proteins interact with the HOPS complex and function in lysosomal delivery.

Zhu GD, Salazar G, Zlatic SA, Fiza B, Doucette MM, Heilman CJ, Levey AI, Faundez V, L'hernault SW

Yeast and animal homotypic fusion and vacuole protein sorting (HOPS) complexes contain conserved subunits, but HOPS-mediated traffic in animals might require additional proteins. Here, we demonstrate that SPE-39 homologues, which are found only in animals, are present in RAB5-, RAB7-, and RAB11-positive endosomes where they play a conserved role in lysosomal delivery and probably function via their interaction with the ... [more]

Mol. Biol. Cell Feb. 01, 2009; 20(4);1223-40 [Pubmed: 19109425]

Throughput

  • Low Throughput

Curated By

  • BioGRID