BAIT

EMI1

YDR512C

Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs

GO Process (3)

GO Function (0)

GO Component (0)

Gene Ontology Biological Process

ascospore formation [IMP]

mitochondrial respiratory chain complex assembly [IMP]

positive regulation of transcription from RNA polymerase II promoter [IMP]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

PREY

CMR1

YDL156W

DNA-binding protein with preference for UV-damaged DNA; protein sequence contains three WD domains (WD-40 repeat); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; potential regulatory target of Mbp1p, which binds to the promoter region; co-localizes with Hos2p in nuclear foci in response to DNA damage by MMS

GO Process (0)

GO Function (1)

GO Component (3)

Gene Ontology Molecular Function

DNA binding [IDA]

Gene Ontology Cellular Component

cytoplasm [IDA]

nuclear chromatin [IDA]

nucleus [IDA]

SGD Alliance of Genome Resources Entrez Gene RefSeq UniprotKB

Saccharomyces cerevisiae (S288c)

Synthetic Growth Defect

A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.

Publication

Dissecting DNA damage response pathways by analysing protein localization and abundance changesÂ during DNA replication stress.

Tkach JM, Yimit A, Lee AY, Riffle M, Costanzo M, Jaschob D, Hendry JA, Ou J, Moffat J, Boone C, Davis TN, Nislow C, Brown GW

Relocalization of proteins is a hallmark of the DNA damage response. We use high-throughput microscopic screening of the yeast GFP fusion collection to develop a systems-level view of protein reorganization following drug-induced DNA replication stress. Changes in protein localization and abundance reveal drug-specific patterns of functional enrichments. Classification of proteins by subcellular destination enables the identification of pathways that respond ... [more]

Unknown Jul. 29, 2012; 0(0); [Pubmed: 22842922]

Quantitative Score

-0.127 [SGA Score]

Throughput

High Throughput

Ontology Terms

phenotype: vegetative growth (APO:0000106)
phenotype: colony size (APO:0000063)

Additional Notes

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CMR1 EMI1	Negative Genetic Negative Genetic Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.	Gallina I (2015)	High	7.46	BioGRID	1112733
CMR1 EMI1	Synthetic Growth Defect Synthetic Growth Defect A genetic interaction is inferred when mutations in separate genes, each of which alone causes a minimal phenotype, result in a significant growth defect under a given condition when combined in the same cell.	Tkach JM (2012)	High	-0.127	BioGRID	721946

Curated By

BioGRID

Interaction Summary