BAIT
AP2B1
adaptor-related protein complex 2, beta 1 subunit
GO Process (1)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
ITSN1
Itsn
intersectin 1 (SH3 domain protein)
GO Process (6)
GO Function (4)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly.
Adaptor protein complex 2 alpha and beta-appendage domains act as hubs for the assembly of accessory protein networks involved in clathrin-coated vesicle formation. We identify a large repertoire of beta-appendage interactors by mass spectrometry. These interact with two distinct ligand interaction sites on the beta-appendage (the "top" and "side" sites) that bind motifs distinct from those previously identified on the ... [more]
PLoS Biol. Sep. 01, 2006; 4(9);e262 [Pubmed: 16903783]
Throughput
- Low Throughput
Curated By
- BioGRID