BAIT
AP2B1
adaptor-related protein complex 2, beta 1 subunit
GO Process (1)
GO Function (3)
GO Component (5)
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
PREY
HSPA8
Hsc70
heat shock 70kDa protein 8
GO Process (10)
GO Function (13)
GO Component (18)
Gene Ontology Biological Process
- ATP catabolic process [IDA, ISO]
- chaperone mediated protein folding requiring cofactor [IDA, ISO]
- chaperone-mediated protein folding [IDA]
- clathrin coat disassembly [ISO]
- negative regulation of fibril organization [ISO]
- negative regulation of transcription, DNA-templated [ISO, ISS]
- positive regulation of mRNA splicing, via spliceosome [ISO]
- protein folding [ISO]
- protein refolding [ISO]
- regulation of cell cycle [ISO]
Gene Ontology Molecular Function- ADP binding [IDA]
- ATP binding [IDA, ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IMP, ISO]
- ADP binding [IDA]
- ATP binding [IDA, ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [IDA, ISO]
- G-protein coupled receptor binding [ISO]
- MHC class II protein complex binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protein binding [IPI]
- receptor binding [IPI]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [IMP, ISO]
Gene Ontology Cellular Component
- Prp19 complex [ISO, ISS]
- blood microparticle [ISO]
- cytoplasm [IDA]
- cytosol [ISO]
- extracellular space [ISO]
- extracellular vesicular exosome [IDA, ISO]
- focal adhesion [ISO]
- intracellular [ISO]
- membrane [ISO]
- myelin sheath [ISO]
- neuron projection [IDA]
- neuronal cell body [IDA]
- nucleus [ISO, ISS]
- protein complex [IDA]
- ribonucleoprotein complex [ISO, ISS]
- synaptic vesicle [IDA]
- terminal bouton [IDA]
- ubiquitin ligase complex [ISO]
Rattus norvegicus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Role of the AP2 beta-appendage hub in recruiting partners for clathrin-coated vesicle assembly.
Adaptor protein complex 2 alpha and beta-appendage domains act as hubs for the assembly of accessory protein networks involved in clathrin-coated vesicle formation. We identify a large repertoire of beta-appendage interactors by mass spectrometry. These interact with two distinct ligand interaction sites on the beta-appendage (the "top" and "side" sites) that bind motifs distinct from those previously identified on the ... [more]
PLoS Biol. Sep. 01, 2006; 4(9);e262 [Pubmed: 16903783]
Throughput
- Low Throughput
Curated By
- BioGRID